Process for producing L-amino acid through fermentation
AssigneeAjinomoto Co., Inc.
InventorsYoshimi Kikuchi, Kazuo Nakanishi, Hiroyuki Kojima
Life of this patent
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Abstract
A DNA encoding aspartokinase III derived from bacteria of the genus Escherichia and having mutation by which feedback inhibition with L-lysine is released is introduced into cells to form transformant bacteria of the genus Escherichia. These bacteria are incubated in an appropriate culture medium. An L-amino acid is produced and accumulated in the culture, and collected from this culture.
Claims79
An isolated nucleic acid molecule encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein the mutations are selected from the group consisting of:
- the aspartic acid at position 202 is substituted with another amino acid,
- the glutamic acid at position 250 is substituted with another amino acid,
- the arginine at position 283 of the mutant is substituted with another amino acid,
- the serine at position 321 is substituted with another amino acid,
- the valine at position 328 is substituted with another amino acid,
- the alanine at position 333 is substituted with another amino acid,
- the serine at position 338 is substituted with another amino acid,
- the threonine at position 344 is substituted with another amino acid,
- the glutamic acid at position 346 is substituted with another amino acid,
- the serine at position 358 is substituted with another amino acid,
- the threonine at position 364 is substituted with another amino acid,
- the leucine at position 374 is substituted with another amino acid,
- the glutamic acid at position 405 is substituted with another amino acid, and
- the asparagine at position 414 is substituted with another amino acid.
An isolated nucleic acid molecule encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein:
- the methionine at position 318 of the mutant is substituted with another amino acid, and
- the glycine at position 323 of the mutant is substituted with another amino acid.
An isolated nucleic acid molecule encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein:
- the leucine at position 325 of the mutant is substituted with another amino acid, and
- the valine at position 347 of the mutant is substituted with another amino acid.
An isolated nucleic acid molecule encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein:
- the glycine at position 323 of the mutant is substituted with another amino acid, and
- the valine at position 347 of the mutant is substituted with another amino acid.
An isolated nucleic acid molecule encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein:
- the leucine at position 325 of the mutant is substituted with another amino acid, and
- the serine at position 345 of the mutant is substituted with another amino acid.
An isolated nucleic acid encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein:
- the glycine at position 323 of the mutant is substituted with another amino acid, and
- the serine at position 358 of the mutant is substituted with another amino acid.
An isolated nucleic acid molecule encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein:
- the methionine at position 318 of the mutant is substituted with another amino acid,
- the serine at position 321 of the mutant is substituted with another amino acid,
- the valine at position 328 of the mutant is substituted with another amino acid,
- the valine at position 349 of the mutant is substituted with another amino acid, and
- the glutamic acid at position 405 of the mutant is substituted with another amino acid.
An isolated nucleic acid molecule encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein:
- the amino acid at position 323 of the mutant is aspartic acid, and
- the amino acid at position 358 of the mutant is leucine.
An isolated nucleic acid molecule encoding a mutant of the aspartokinase set forth in SEQ ID NO:2, wherein:
- the amino acid at position 318 of the mutant is lysine,
- the amino acid at position 321 of the mutant is proline,
- the amino acid at position 328 of the mutant is phenylalanine,
- the amino acid at position 349 of the mutant is glycine, and
- the amino acid at position 405 of the mutant is valine.
Dependencies are inferred from the claim text.