Processes and agents for detecting listerias
AssigneeMerck Patent Gesellschaft mit
InventorsPeter Schubert, Siegfried Neumann, Martina Pawelzik, Winfried Linxweiler, Christa Burger, Gottfried Hofmann, Andreas Bubert, Werner Goebel, Stefan Kohler
Life of this patent
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Abstract
The invention relates to agents and processes for detecting bacteria of the genus Listeria, in particular L. monocytogenes. The agents according to the invention include primers whose sequence is selected from the iap gene of L. monocytogenes. In addition, the agents according to the invention include peptides whose sequence is selected from the p60 protein and which are suitable for producing specific antibodies for the immunological detection of L. monocytogenes.
Claims6
An isolated DNA molecule consisting of a nucleotide sequence selected from one of the formulae (Va)-(Vh),
- wherein
- X.sup.1 and X.sup.2 are each, independently, hydrogen or 1-20 additional nucleotides,
- X.sup.1 AATATGAAAAAAGCX.sup.2 (SEQ ID NO:1) (Va),
- X.sup.1 GCTTCGGTCGCGTAX.sup.2 (SEQ ID NO:2) (Vb),
- X.sup.1 ACAGCTGGGATTGCX.sup.2 (SEQ ID NO:3) (Vc),
- X.sup.1 ACTCGTAACACAGCTX.sup.2 (SEQ ID NO:4) (Vd),
- X.sup.1 TAACAGCAATTCAAGX.sup.2 (SEQ ID NO:5(Ve),
- X.sup.1 CTGAGGTAGCGAGCX.sup.2 (SEQ ID NO:6) (Vf),
- X.sup.1 AGCACTCCAGTTGTTAX.sup.2 (SEQ ID NO:7) (Vg), or
- X.sup.1 GCAGTTTCTAAACCTX.sup.2 (SEQ ID NO:8 (Vh),
- or a DNA segment fully complementary to said DNA molecule consisting of the sequence of formulae (Va)-(Vh), wherein said DNA molecule is useful as a primer for amplifying an iap (invasion-associated protein) gene.
Dependencies are inferred from the claim text.