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AssigneesCroptech Development Corporation, Virginia Tech Intellectual Properties, Inc.
InventorsDavid N. Radin, Carole L. Cramer, Karen K. Oishi, Deborah L. Weissenborn
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The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants having recombinant expression constructs comprising human hGC and IDUA nucleotide sequences produced enzymatically active modified human glucocerebrosidase and human .alpha.-L-iduronidase. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety of purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storage diseases.
A method for producing a lysosomal enzyme which is enzymatically active, comprising:
A recombinant expression construct comprising a nucleotide sequence encoding a lysosomal enzyme and a promoter that regulates the expression of the nucleotide sequence in a plant cell.
A plasmid CTPro1:hGC:FLAG having the ATCC accession number 97277.
A plasmid pCT22 having the ATCC accession nunber 97701.
A plasmid pCT54 having the ATCC accession number 97770.
A transgenic plant or plant cell capable of producing a lysosomal enzyme which is enzymatically active, which transgenic plant or plant cell is transformed or transfected with a recombinant expression construct comprising a nucleotide sequence encoding a lysosomal enzyme and a promoter that regulates expression of the nucleotide sequence in the transgenic plant or plant cell.
A seed of plant line hGC X-11, which seed has the ATCC Accession No. 97275.
A seed of plant line hGC X-27, which seed has the ATCC Accession No. 97276.
A seed of plant line CT40-9, which seed has the ATCC Accession No. 97700.
A lysosomal enzyme which is enzymatically active and is produced according to a process comprising:
Dependencies are inferred from the claim text.