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US 5,928,879 AGranted
Method of diagnosing human granulocytic Ehrlichiosis
AssigneesRegents of the University of Minnesota, The Regents of the University of California, University of Maryland at Baltimore
InventorsJ. Stephen Dumler, Ulrike G. Munderloh, John Madigan, Jesse Goodman, Timothy J. Kurtti
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Abstract
Methods for the in vitro cultivation, propagation, and production of antigens of Ehrlichia phagocytophila genogroup granulocytic Ehrlichiae, including Ehrlichia equi, in Ixodes scapularis tick cell culture and in human HL60 promyelocytic leukemia cell culture.
Claims5
A method of diagnosing human granulocytic ehrlichiosis (HGE) in a human patient comprising the steps of:
- (a) providing an infected HL60 cell culture, wherein the HL60 cell culture is infected with an Ehrlichia phagocytophila genogroup organism,
- (b) incubating the infected cells with antiserum from said patient, and
- (c) diagnosing HGE in said patient by detecting immunoreaction of the antiserum from said patient with the infected HL60 cell culture.
Dependencies are inferred from the claim text.