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AssigneeThe Secretary of State for Defence in her Britannic Majesty's Government of the United Kingdom of Great Britain & North Ireland of Defence Evaluation & Research Agency
InventorsStephen Nicklin, Peter Roland Binks, Neil Charles Bruce, Christopher Edward French
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An enzyme capable of catalyzing the removal of nitrite from pentaerythritol tetranitrate (PETN) is provided. The enzyme (known as PETN reductase enzyme) is produced by culturing a novel strain of the Enterobacter cloacae bacterium isolated from nature. The strain designated PB2 has been deposited as NCIMB 40718. The amino acid sequence of the enzyme and the genetic sequence which encodes for this enzyme have also been determined. A PETN reductase enzyme encoded by the on gene is provided. A method for producing PETN reductase enzyme in large quantities and methods of bioremediation using the enzyme so produced are also provided. Additionally there is provided a method of detecting the presence of PETN in a sample together with a biosensor for use in such a method.
A PETN reductase enzyme which catalyses the removal of nitrite from PETN characterised in having the amino acid sequence as set forth in SEQ ID NO:
A method of detecting PETN in a sample, comprising subjecting the sample to a reaction involving the removal of nitrite from PETN, the reaction being carried out in the presence of NADPH and PETN reductase enzyme, having the amino acid sequence as set forth in SEQ ID NO:
An Enterobacter cloacae bacterial strain referred to as "PB2" and deposited as NCIMB 40718, and mutants thereof capable of producing enzymic activity which degrades PETN in the presence of NADPH.
Dependencies are inferred from the claim text.