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US 5,925,565 AGranted
Internal ribosome entry site, vector containing it and therapeutic use
AssigneeInstitut National de la Sante et de la Recherche Medicale
InventorsClarisse Berlioz, Sandrine Jacquemoud, Christophe Torrent, Jean-Luc Darlix
Life of this patent
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Abstract
A novel internal ribosome entry cite and a novel region for the encapsidation of a retrotransposon and murine VL30s in particular are disclosed. A vector and a eukaryotic cell containing said cite and region, and their therapeutical or prophylactic use are also disclosed.
Claims20
A polycistronic vector for the expression of at least two polynucleotide sequences comprising
- a promoter operably linked to
- a nucleotide sequence comprising elements encoding two or more proteins, and
- a DNA fragment of a VL30 retrotransposon comprising at least 100 bp to 1.5 kbp of a VL30 retrotransposon located between the elements of said nucleotide sequence, wherein said DNA fragment acts as an internal ribosome entry site.
A polycistronic vector for the expression of at least three polynucleotide sequence comprising
- a promoter operably to
- a nucleotide sequence comprising elements encoding three or more proteins, and
- DNA fragments of two or more different VL30 retrotransposons each comprising at least 100 bp to 1.5 kbp of a VL30 retrotransposons located between each of the elements of said nucleotide sequence, wherein said DNA fragments act as internal ribosome entry sites.
Dependencies are inferred from the claim text.