Endo-.beta.-galactosidase
AssigneeNew York Blood Center, Inc.
InventorsJack Goldstein, Alex Zhu, Lin Leng
Life of this patent
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Abstract
The present invention relates to purified and isolated nucleic acid encoding the endo-.beta.-galactosidase from Flavobacterium keratolyticus (referred to as "ENDO-A"), and to purified ENDO-A protein. The endo-.beta.-galactosidase of the invention may be used in a process which enzymatically de-antigenizes human erythrocytes bearing A.sub.1 antigen. The resulting erythrocytes may be transfused into individuals who would be otherwise unable to tolerate a transfusion of type A.sub.1 blood.
Claims16
A purified and isolated nucleic acid encoding an endo-.beta.-galactosidase from Flavobacterium keratolyticus.
A purified and isolated nucleic acid encoding ENDO-A, and having a sequence as set forth in FIG. 2 (SEQ ID NO:1).
A purified and isolated nucleic acid encoding ENDO-A, as prepared from the vector pCR-ED, as deposited with the American Type Culture Collection and assigned accession number 97699.
A purified and isolated nucleic acid encoding ENDO-A, wherein ENDO-A has an amino acid sequence as set forth in FIG. 2 (SEQ ID NO:2).
A method of producing endo-.beta.-galactosidase comprising (i) introducing a nucleic acid encoding ENDO-A into a suitable host cell;
- (ii) culturing the host cell produced in step (i) under conditions compatible with expression of ENDO-A;
- and (iii) purifying ENDO-A from the culture produced in step (ii).
Dependencies are inferred from the claim text.