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US 5,925,356 AGranted
Method of isolating cucurbitacin
OwnerVen Subbiahinventor
Life of this patent
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Abstract
A method of isolating and purifying cucurbitacin is described. The method involves providing a cucurbitacin-containing liquid obtained from plant matter containing cucurbitacin, which liquid is then sequentially extracted with a non-polar solvent and then a moderately polar solvent. In a preferred embodiment, the cucurbitacin is purified by flash-column chromatography.
Claims7
A method of fractionally isolating a cucurbitacin from Cucurbita andreana, comprising the steps of:
- (a) providing a cucurbitacin-containing liquid obtained from Cucurbita andreana plant matter containing cucurbitacin, whereby the cucurbitacin-containing liquid is obtained by compressing the Cucurbita andreana plant matter containing cucurbitacin to yield a liquid containing cucurbitacin;
- (b) extracting the cucurbitacin-containing liquid with at least one non-polar solvent to provide a cucurbitacin-containing solution;
- (c) extracting the solution produced in step (b) with at least one moderately polar solvent to provide an organic phase comprising a mixture of cucurbitacins;
- (d) separating the mixture of cucurbitacins from step (c) from the organic phase of step (c) using silica gel column chromatography;
- (e) eluting the separating column of step (d) with a mixture of solvents to yield a composition consisting of Cucurbitacin B;
- and
- (f) collecting the composition consisting of Cucurbitacin B.
4 more elements
A method according to claim whereby said separating step (d) is carried out using flash column chromatography.
Dependencies are inferred from the claim text.