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US 5,922,597 AGranted
Ex vivo culture of stem cells
AssigneeRegents of the University of Minnesota
InventorsCatherine M. Verfaillie, Philip B. McGlave, Jeffrey S. Miller
Life of this patent
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Abstract
A method for the culture of hematopoietic cells is provided comprising culturing said cells in stromal conditioned medium comprising one or more cytokines selected from the group consisting of MIP-1.alpha., IL-3, SCF, BB10010 and PF-4, so that the ability of the stem cells to self-replicate and differentiate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
Claims11
A method for culturing hematopoietic cells, comprising:
- culturing a population of human hematopoietic cells comprising stem cells or committed progenitor cells in a stromal conditioned medium in the absence of stromal cells, wherein the medium comprises an effective added amount of one or more cytokines selected from the group consisting of MIP-1.alpha., IL-3, SCF, BB10010 and PF-4, so that the ability of the stem cells to self-replicate and differentiate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
Dependencies are inferred from the claim text.