Biosensing devices which produce diffraction images
AssigneeKimberly-Clark Worldwide, Inc.
InventorsDennis S. Everhart, Michael Grunze, Rosann Marie Kaylor, Friderike Karolin Deseree Morhard
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Abstract
The present invention provides an inexpensive and sensitive device and method for detecting and quantifying analytes present in a medium. The device comprises a metalized film upon which is printed a specific, predetermined pattern of a analyte-specific receptors. Upon attachment of a target analyte to select areas of the plastic film upon which the receptor is printed, diffraction of transmitted and/or reflected light occurs via the physical dimensions and defined, precise placement of the analyte. A diffraction image is produced which can be easily seen with the eye or, optionally, with a sensing device.
Claims52
A biosensor comprising:
- a polymer film coated with metal;
- and
- a self-assembling monolayer printed onto the polymer film wherein the self-assembling monolayer has a receptive material thereon that is specific for an analyte;
- wherein the self-assembling monolayer is printed in a first, non-diffracting pattern such that when the biosensor binds an analyte, the biosensor diffracts transmitted light to form a second pattern, wherein the second pattern is a diffraction pattern;
- and
- further wherein the analyte has a size of the same order as the wavelength of the transmitted light thereby resulting in the diffraction of the transmitted light.
A method of making a biosensor comprising printing a pattern of self-assembling monolayers with a receptive material on a polymer film coated with metal, wherein the self-assembling monolayer is printed in a pattern such that when the biosensor binds an analyte, the biosensor diffracts transmitted light to form a diffraction pattern.
A method of making a biosensor comprising printing a pattern of self-assembling monolayers with a receptive material on a polymer film coated with metal;
- wherein the self-assembling monolayer is printed in a first, non-diffracting pattern such that when the biosensor binds an analyte, the biosensor diffracts transmitted light to form a second pattern, wherein the second pattern is a diffraction pattern;
- and
- further wherein the analyte has a size of the same order as the wavelength of the transmitted light thereby resulting in the diffraction of the transmitted light.
A method of detecting an analyte in a medium comprising:
- contacting the medium suspected of containing the analyte with a biosensing device, the biosensing device comprising:
- a polymer film coated with metal;
- and
- a self-assembling monolayer printed in a first, non-defracting pattern onto the polymer film wherein the self-assembling monlayer has a receptive material thereon that is specific for the analyte;
- and
- transmitting a light through the polymer film;
- and
- detecting the presence of the analyte bound to the receptive material by detecting a second pattern formed form by the diffraction of the transmitted light;
- further wherein the analyte has a size of the same order as the wavelength of the transmitted light thereby resulting in the diffraction of the transmitted light.
Dependencies are inferred from the claim text.