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US 5,922,545 AGranted
In vitro peptide and antibody display libraries
AssigneeAffymax Technologies N.V.
InventorsLarry C. Mattheakis, William J. Dower
Life of this patent
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Abstract
Improved methods and novel compositions for identifying peptides and single-chain antibodies that bind to predetermined receptors or epitopes. Such peptides and antibodies are identified by improved and novel methods for affinity screening of polysomes displaying nascent peptides.
Claims4
A method for identifying multiple binding specificities of single chain antibodies, said method comprising:
- contacting under suitable binding conditions a multiplicity of antigen species with a polysome library displaying nascent peptides having a single chain antibody segment;
- and
- separating polysomes bound to the antigen species from polysomes not bound to the antigen species;
- synthesizing cDNA from the separated bound polysomes, thereby identifying single chain antibodies which bind to at least one of the antigen species present in the multiplicity of antigens.
1 more element
Dependencies are inferred from the claim text.