Equine herpes virus glycoproteins
AssigneeThe University of Melbourne
InventorsBrendan Scott Crabb, Michael Justin Studdert
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Abstract
Equine rhinopneumonitis and equine abortion are horse diseases caused by two distinct but antigenically related viruses designated as equine herpes virus 4 (EHV4) and equine herpes virus 1 (EHV1). Glycoprotein G (gG) epitopes of EHV1 and EHV4 of this invention allow for accurate, type-specific diagnosis of the infections despite extensive antigenic cross-reactivity between the two viruses.
Claims37
An isolated envelope glycoprotein G (gG) of equine herpesvirus (EHV) that elicits a type-specific response to equine herpesvirus 4 (EHV4), wherein said envelope glycoprotein G is EHV4 gG.
An EHV4 epitope that elicits a type-specific response to EHV4, comprising the amino acid sequence of SEQ ID NO:4 or a subsequence of SEO ID NO:4 that elicits a type-specific response to EHV4.
An EHV4 epitope that elicits a type-specific response to EHV4, comprising the amino acid sequence of SEQ ID NO:5 or a subsequence of SEQ ID NO:5 that elicits a type-specific response to EHV4.
Plasmid vector pEG4, var.
An EHV1 epitope that elicits a type-specific response to EHV1, consisting of the amino acid sequence of SEQ ID NO:6, or a subsequence of SEQ ID NO:6 that elicits a type-specific response to EHV1.
An EHV1 epitope that elicits a type-specific response to EHV1, consisting of the amino acid sequence of SEQ ID NO:7 or a subsequence of SEQ ID NO:7 that elicits a typespecific response to EHV1.
Plasmid vector pEG1var.
An immunological test kit for screening for EHV4 and EHV1 infection of a horse, comprising as capture antigens (1) an envelope glycoprotein comprising an EHV4 epitope that elicits a type-specific response to EHV4 and (2) an envelope glycoprotein comprising an EHV1 epitope that elicits a type-specific response to EHV1.
A hybridization nucleic acid probe specific for EHV1 gG gene selected from the group consisting of:
- a probe consisting of the nucleotides encoding the amino acid sequence of SEQ ID NO:6 or a subsequence of SEQ ID NO:6 that elicits a type-specific response to EHV1, and
- a probe consisting of the nucleotides encoding the amino acid sequence of SEQ ID NO:7 or a subsequence of SEQ ID NO:7 that elicits a type-specific response to EHV1.
A hybridization nucleic acid probe specific for EHV4 gG gene selected from the group consisting of:
- a probe encoding the amino acid sequence of SEQ ID NO:4 or a subsequence of SEQ ID NO:4 that elicits a type-specific response to EHV4, and
- a probe encoding the amino acid sequence of SEQ ID NO:5 or a subsequence of SEQ ID NO:5 that elicits a type-specific response to EHV4.
An isolated envelope glycoprotein G (gG) of equine herpesvirus (EHV) that elicits a type-specific response to equine herpesvirus 4 (EHV4), wherein the envelope glycoprotein G is EHV4 gG encoded by the sequence of the exogenous DNA structural sequence of plasmid vector pEG4.1.
An EHV4 epitope that elicits a type-specific response to EHV4, wherein the epitope is encoded by the exogenous DNA structural sequence of plasmid vector pEG4.var.
An EHV4 epitope that elicits a type-specific response to EHV4, comprising a subsequence of SEQ ID NO:2, wherein the subsequence elicits a type-specific response to EHV4, and wherein the epitope does not comprise full-length EHV4 gG.
An EHV1 epitope that elicits a type-specific response to EHV1, comprising the amino acid sequence of SEQ ID NO:6 or a subsequence of SEQ ID NO:6 that elicits a type-specific response to EHV1, wherein the EHV1 epitope is not the full-length EHV1 gG.
An EHV1 epitope that elicits a type-specific response to EHV1, comprising the amino acid sequence of SEQ ID NO:7 or a subsequence of SEQ ID NO:7 that elicits a type-specific response to EHV1, wherein the EHV1 epitope is not the full-length EHV1 gG.
Dependencies are inferred from the claim text.