Genetic control of plant hormone levels and plant growth
AssigneeMichigan State University Board of Trustees
InventorsRobert S. Bandurski, Jedrzej Bogumil Szerszen, deceased, Krzysztof Szczyglowski
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Abstract
Plant growth and plant growth habit can be controlled without the application of exogenous plant hormones or hormone mimetics using the nucleic acid sequences and methods provided. UDP-Glucose: Indol-3-ylacetyl-glucosyl transferase (IAGlu Transferase) amino acid sequence and nucleic acid coding sequences for this enzyme, specifically exemplified for Zea mays, are provided. Nucleic acid constructs directing the expression of IAGlu Transferase and the expression of antisense RNA specific therefor allows the control of growth habit and plant size in transgenic plants containing such nucleic acid constructs.
Claims21
A non-naturally occurring nucleic acid molecule comprising a nucleotide sequence, wherein said nucleotide sequence encodes a UDP-glucose indol-3-ylacetylglucosyl transferase (IAGlu Transferase), said IAGlu transferase having an amino acid sequence as given in SEQ ID NO:2.
An antisense construct comprising:
- a promoter portion, said promoter being fictional in a plant cell;
- and an iaglu antisense portion, said promoter portion being operably linked to said iaglu antisense portion;
- and said antisense portion oriented with respect to said promoter portion such that RNA produced is complementary in nucleotide sequence and capable of binding or hybridizing to mRNA encoding UDP-glucose indol-3ylglucosyl transferase, wherein said iaglu antisense portion has a nucleotide sequence as given in at least 15 contiguous nucleotides of SEQ ID NO:1.
A transgenic plant comprising of an iaglu antisense construct comprising;
- a promoter portion, said promoter being functional in a plant cell;
- and an iaglu antisense portion, said promoter portion being operably linked to said iaglu antisense portion;
- and said antisense portion oriented with respect to said promoter portion such that RNA produced is complementary in nucleotide sequence and capable of binding or hybridizing to mRNA encoding IAGlu Transferase, wherein said antisense construct comprises a nucleotide sequence of at least 15 bp as given in SEQ ID NO:1.
Dependencies are inferred from the claim text.