Derivatization of solid supports and methods for oligomer synthesis
AssigneeAffymetrix, Inc.
InventorsSteven A. Sundberg, Jeffrey W. Jacobs, John R. Schullek, Glenn H. McGall, Andrei Mirzabekov, Edward Timofeey, David Fujimoto, Christopher P. Holmes, Hua Yang
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Abstract
Methods and derivatized supports which are useful in solid-phase synthesis of peptides, oligonucleotides or other small organic molecules as well as arrays of ligands. The methods provide means to control the functional site density on a solid support. Some of the derivatized supports are polymer-coated or glycan-coated. Other methods for regenerating the surface of a used ligand array are also provided.
Claims7
A method of preparing a polymer-coated support for use in solid-phase synthesis using in situ polymerization, comprising:
- (a) preparing a mixture comprising monomers and a polymerization initiator to initiate polymerization of the monomers;
- (b) contacting a support derivatized with a derivatizing agent having a substrate attaching group attached to the support and a reactive site distal to the support with said mixture to form a polymer linked to the support via the reactive site on the derivatizing agent.
Dependencies are inferred from the claim text.