Isolation and characterization of allergen-binding cells for diagnosis of hypersensitivity
AssigneeMiltenyi Biotec GmbH
InventorsJohannes Irsch, Stefan Miltenyi, Andreas Radbruch
Life of this patent
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Abstract
Methods and compositions are provided for the diagnosis of allergen hypersensitivity in a patient. Rare, allergen-specific cells are enriched from a complex cell population, e.g. a patient blood sample. The percentage of blood cells that bind to a particular allergen is less than 0.01%. The allergen-specific cell population is enriched by magnetic cell sorting. In normal blood, the allergen-binding cells are primarily B-cells expressing CD19 and CD21. In blood from allergic patients, an additional population of effector cells, e.g. basophilic granulocytes is labeled by the allergen.
Claims6
A method of producing allergen-specific monoclonal antibody, the method comprising:
- a. combining a hematopoietic cell sample with a test allergen directly or indirectly coupled to a magnetic microparticle;
- b. passing said cells through a magnetic field;
- c. removing unbound cells;
- and
- d. collecting bound cells in the substantial absence of said magnetic field to provide an enriched cell sample comprising allergen binding cells;
- e. immortalizing B-cells in said enriched cell sample;
- and
- f. screening said immortalized B-cells for cells that produce allergen-specific antibody.
Dependencies are inferred from the claim text.