Method for increasing storage lipid content in plant seed
AssigneesMitsubishi Corporation, Mitsubishi Chemical Corporation
InventorsMakoto Murase, Junko Murase, Mari Iwabuchi, Takahiko Hayakawa, Jun Imamura
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Abstract
In order to obtain a crop which provides an increased storage lipid content in its seed, the activity of cytosolic pyruvate kinase of a seed is substantially lowered as compared with the endogenous activity of pyruvate kinase in the plant seed. Preferably, the activity of phosphoenolpyruvate carboxylase is substantially lowered as compared with the endogenous activity of phosphoenolpyruvate carboxylase in the seed. Thus the storage lipid content in the plant seed is increased.
Claims13
A method for increasing a storage lipid content in a seed of a plant, comprising the step of:
- introducing into the plant a DNA having a sequence coding for pyruvate kinase or a part thereof, wherein by an antisense method or a co-suppression method, expression of cytosolic pyruvate kinase in cells of the seed is suppressed, thereby substantially lowering an activity of cytosolic pyruvate kinase in the seed of the plant as compared with an endogenous activity of cytosolic pyruvate kinase in the seed of the plant, to thereby increase the storage lipid content of the seed.
Dependencies are inferred from the claim text.