Structural gene for membrane-bound aldehyde dehydrogenase

An isolated structural gene for a membrane-bound aldehyde dehydrogenase, which codes for the amino acid sequence as shown in FIG. 3.

An isolated structural gene for the membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said isolated structural gene having a cleavage map as shown in FIG. 1.

An isolated DNA consisting essentially of a sequence of DNA bases coding for the amino acid sequence for membrane-bound aldehyde dehydrogenase shown in FIG. 3.

An isolated DNA consisting essentially of the sequence of DNA bases shown in FIG. 3.

A plasmid for expression of membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said plasmid containing the isolated structural gene for membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter and a promoter therefor, said isolated structural gene having a molecular size of about 3.6 kb, and having a restriction enzyme cleavage map as shown in FIG. 1, said promoter being a promoter for .beta.-galactosidase of Escherichia coli.

Acetic acid bacteria transformed by a plasmid for expression of membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said plasmid containing the isolated structural gene for membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter and a promoter therefor, said isolated structural gene having a molecular size of about 3.6 kb and having a restriction enzyme cleavage map as shown in FIG. 1, said promoter being a promoter for .beta.-galactosidase of Escherichia coli.

In the method of acetic acid fermentation, the improvement comprising using a strain belonging to the genus Acetobacter or Gluconobacter transformed by a plasmid for expression of membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said plasmid containing the isolated structural gene for membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter and a promoter therefor, said purified structural gene having a molecular size of about 3.6 kb and having a restriction enzyme cleavage map as shown in FIG. 1, said promoter being a promoter for .beta.-galactosidase of Escherichia coli.

A plasmid for expression of membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said plasmid containing the isolated structural gene for membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter and a promoter therefor, said isolated structural gene having a molecular size of about 3.6 kb, and having a base sequence as shown in FIG. 3, said promoter being a promoter for .beta.-galactosidase of Escherichia coli.

Acetic acid bacteria transformed by a plasmid for expression of membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said plasmid containing the isolated structural gene for membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter and a promoter therefor, said isolated structural gene having a molecular size of about 3.6 kb and having a base sequence as shown in FIG. 3, said promoter being a promoter for .beta.-galactosidase of Escherichia coli.

In the method of acetic acid fermentation, the improvement comprising using a strain belonging to the genus Acetobacter or Gluconobacter transformed by a plasmid for expression of membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said plasmid containing the isolated structural gene for membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter and a promoter therefor, said purified structural gene having a molecular size of about 3.6 kb and having a base sequence as shown in FIG. 3, said promoter being a promoter for .beta.-galactosidase of Escherichia coli.