Materials and methods for the immobilization of bioactive species onto polymeric substrates

A material having immobilized bioactive species comprising:

• a support member comprised of a polytetrafluoroetylene material;
• a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ
• a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer;
• and
• at least one type of bioactive species attached to the second layer.

A material having immobilized bioactive species comprising:

• a hydrophobic support member;
• a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ;
• a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer;
• and
• at least one type of bioactive species attached to the second layer.

A material having immobilized bioactive species comprising:

• a support member;
• a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-linked to itself in situ;
• a plurality of layers attached to one another wherein each layer is comprised of at least one species of a hydrophilic polymer and wherein at least one layer of the plurality of layers is covalently attached to the first layer;
• and
• at least one type of bioactive species attached to at least one layer of the plurality of layers.

A method of making a material having immobilized bioactive species which comprises:

• providing a hydrophobic support member;
• attaching a first layer comprised of polymeric surfactants to the support member non-covalently;
• cross-linking the polymeric surfactants in situ with a cross-linking agent;
• attaching a second layer comprised of at least one type of hydrophilic polymer to the first layer convalently;
• and
• attaching a bioactive species to the second layer.

A material having immobilized bioactive species comprising:

• a hydrophobic support member;
• a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ;
• and
• at least one type of bioactive species attached to the first layer.

A material having immobilized bioactive species comprising:

• a hydrophobic support member;
• a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ;
• a plurality of layers attached to one another wherein each layer is comprised of at least one species of a hydrophilic polymer and wherein at least one layer of the plurality of layers is covalently attached to the first layer;
• and
• at least one type of bioactive species attached to at least one layer of the plurality of layers.