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US 5,912,177 AGranted
Stem cell immobilization
AssigneeCommon Services Agency
InventorsMarc Leighton Turner, William Gerrard Murphy
Life of this patent
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AssigneeCommon Services Agency
InventorsMarc Leighton Turner, William Gerrard Murphy
Estimating the term…
Haematopoietic stem cells (which term includes early progenitor cells) are immobilized on a substrate coated with a fibrin matrix and including a substance capable of both binding to the fibrin matrix and also having an RGD amino acid sequence for binding to the stem cells. The substance may be fibronectin or thrombospondin. The substrate is generally in the form of a closed bag formed of a carbon dioxide-permeable and oxygen-permeable plastics material which allows culturing of the stem cells. The cultured stem cells may re-engraft a patient following chemotherapy or to correct haemotological deficiencies. Stem cells may be harvested from peripheral blood onto the coated substrate. The stem cells in contact with the coated substrate are good candidates for gene therapy to introduce a heterologous gene e.g. employing a transfection vector.
A system for selectively immobilizing and culturing stem cells onto the inner surface of a flexible container, the system comprising a closed container formed of a flexible plastic material which is permeable to carbon dioxide and oxygen, the container including a substrate having a coating disposing a fibrin matrix, together with a substance capable of binding to the fibrin matrix and having an RGD amino acid sequence for binding to the stem cells, wherein the container is configured to allow stem cell culture to adhere to the inner surface of the flexible container.
A method of harvesting stem cells from peripheral or cord blood, the method comprising the steps of introducing the blood into a closed container formed of a flexible plastic material which is permeable to carbon dioxide and oxygen, disposing in said container a substrate having a coating including a fibrin matrix, together with a substance capable of binding to the fibrin matrix and having an RGD amino acid sequence for binding to the stem cells, such that the stem cells are selectively immobilized on the substrate and harvested from the blood, and adhering said stem cells to the inner surface of the flexible container.
A method of storing and preserving stem cells on the inner surface of a flexible container, which comprises the steps of storing the stem cells in a closed container formed of a flexible plastic material which is permeable to carbon dioxide and oxygen, the closed container including a substrate having a coating including a fibrin matrix, together with a substance capable of binding to the fibrin matrix and having an RGD amino acid sequence for binding to the stem cells such that the stem cells are immobilized on the substrate within the container, and adhering said stem cells to the inner surface of the flexible container.
A method of transfecting stem cells which comprises maintaining the stem cells in a closed container formed of a flexible carbon dioxide-permeable and oxygen-permeable plastic material, the container including a substrate having a coating including a fibrin matrix, together with a substance capable of binding to the fibrin matrix and having an RGD amino acid sequence for binding to the stem cells, such that the stem cells are immobilized on the substrate within the container, and adhering said stem cells to the inner surface of the flexible container;
A method of culturing stem cells in vitro onto the inner surface of a flexible container, which comprises the steps of culturing the stem cells in a closed container formed of a flexible carbon dioxide-permeable and oxygen-permeable plastics material, the container including a substrate having a coating disposing a fibrin matrix, together with a substance capable of binding to the fibrin matrix and having an RGD amino acid sequence for binding to the stem cells, such that the stem cells are immobilized and cultured on the substrate within the container, and adhering said stem cells to the inner surface of the flexible container.
Dependencies are inferred from the claim text.