Autocatalytic cleavage site and use thereof in a protein expression vector
AssigneeWisconsin Alumni Research Foundation
InventorsAnn C. Palmenberg, Michael A. Hoffman, Harry Hahn, Lee R. Martin
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Abstract
A nucleic acid construct comprising at least two copies of a nucleic acid sequence encoding an autocatalytic peptide cleavage site is disclosed. This site preferably comprises the amino acid sequence DX(D,E)XNPGP. A method of exposing an animal to an antigenic amino acid sequence is also disclosed.
Claims9
A nucleic acid construct comprising at least two copies of a nucleic acid sequence encoding an autocatalytic peptide cleavage site, wherein the site comprises the amino acid sequence X(Y,F,I)XXXXXXXD(V,I)(D,E)XNPGP (SEQ ID NO:13) and wherein the construct is a part of a replication competent picornavirus viral sequence and wherein the copies are located at the site of a naturally occurring autocatalytic ceavage site.
Dependencies are inferred from the claim text.