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US 5,912,132 AGranted
Identification of ligands by selective amplification of cells transfected with receptors
AssigneeACADIA Pharmaceuticals Inc.
InventorMark Robert Brann
Life of this patent
Estimating the term…
Abstract
The invention is directed to a method for identifying substances acting as ligands for transfected receptors by using transfected markers to measure receptor/ligand interactions.
Claims14
A test kit for detecting a substance capable of acting as a ligand, the kit comprising
- (a) a culture of frozen cells which comprise cells transfected with DNA coding for a receptor which increases or decreases cell amplification in response to a ligand, said transfected cells also comprising a transfected marker of cell amplification and which cell culture also comprises non-transfected cells which do not comprise said transfected marker;
- (b) a medium for growing cells;
- and
- (c) a reagent for detecting the presence and quantity of the marker.
1 more element
A test kit for detecting a substance capable of acting as a ligand, the kit comprising,
- (a) a culture of frozen cells which comprises cells transfected with DNA coding for a first receptor which increases or decreases cell amplification in response to a ligand, said transfected cells also comprising a transfected marker of cell amplification and which cell culture also comprises non-transfected cells which do not comprise said transfected marker,
- (b) a culture of frozen cells which comprises cells transfected with DNA coding for a second receptor which increases or decreases cell amplification in response to a ligand, said transfected cells also comprising a transfected marker of cell amplification and which cell culture also comprises non-transfected cells which do not comprise said transfected marker,
- (c) a medium for growing the cells, and
- (d) a reagent for detecting the presence and quantity of the marker.
1 more element
Dependencies are inferred from the claim text.