Cloning, expression and diagnosis of human cytochrome P450 2C19: the principal determinant of s-mephenytoin metabolism

A diagnostic kit comprising:

• a forward primer sufficiently complementary with a first subsequence of the antisense strand of a double-stranded 2C19 genomic DNA sequence to hybridize therewith, and a reverse primer sufficiently complementary with a second subsequence of the sense strand of the 2C19 genomic sequence to hybridize therewith;
• wherein the first subsequence is upstream of nucleotide 681 of the coding region, and second subsequence is downstream of nucleotide 681 of the coding region,
• wherein each nucleotide in the coding region of the genomic DNA sequence is designated the same number as the corresponding nucleotide in the coding region of the cDNA sequence in FIG. 2, clone 11a (SEQ. ID. No. 2), when the genomic DNA and the cDNA sequence are maximally aligned, and the nucleotides in the coding region of the cDNA sequence are assigned successive numbers starting at 1 for the A residue of the initiating methionine codon wherein said subsequences are within a segment bordered by the 5' end of the third and 3' end of the fifth intronic regions of the 2C19 genomic DNA sequence.

A diagnostic kit comprising:

• a forward primer sufficiently complementary with a first subsequence of the antisense strand of a double-stranded 2C19 genomic DNA sequence to hybridize therewith, and a reverse primer sufficiently complementary with a second subsequence of the sense strand of the 2C19 genomic sequence to hybridize therewith;
• wherein the first subsequence is upstream of nucleotide 636 of the coding region, and second subsequence is downstream of nucleotide 636 of the coding region,
• wherein each nucleotide in the coding region of the genomic DNA sequence is designated the same number as the corresponding nucleotide in the coding region of the cDNA sequence in FIG. 2, clone 11a (SEQ. ID. No. 2), when the genomic DNA and the cDNA sequence are maximally aligned, and the nucleotides in the coding region of the cDNA sequence are assigned successive numbers starting at 1 for the A residue of the initiating methionine codon wherein said subsequences are within a segment bordered by the 5' end of the third and 3' end of the fourth intronic regions of the 2C19 genomic DNA sequence.