Back to results
US 5,911,998 AGranted
Method of producing a virus vaccine from an African green monkey kidney cell line
AssigneesDyncorp, National Institutes of Health
InventorsLouis Potash, Robert M. Chanock, Robert H. Purcell, Albert Z. Kapikian
Life of this patent
Estimating the term…
Abstract
A method for producing novel African Green Monkey Kidney (AGMK) cell lines is taught. These cell lines which are free of viable adventitious microbial agents are useful as substrates for viruses and for the preparation of viral vaccines.
Claims4
A method of producing a vaccine for a virus selected from the group consisting of rotaviruses, enteroviruses, polioviruses, respiratory viruses, and hepatitis A viruses comprising the steps of:
- (i) producing said virus using a cell line isolated from the kidney of an African Green Monkey, which cell line is substantially free of viable adventitious microbial agents and is able to support the growth of rotaviruses, astroviruses, enteroviruses, polioviruses, respiratory viruses, and hepatitis A viruses, by a method comprising:
- (a) inoculating said cell line with an inoculum of said virus;
- (b) incubating said inoculated cell line to permit proliferation of said virus;
- and
- (c) harvesting the virus resulting from step (c);
- and
- (ii) preparing a vaccine from the harvested virus.
4 more elements
Dependencies are inferred from the claim text.