Low background multi-well plates with greater than 864 wells for fluorescence measurements of biological and biochemical samples
AssigneeAurora Biosciences Corporation
InventorsPeter J. Cassin, Alec Tate Harootunian, Andrew A. Pham, Roger Y. Tsien
Life of this patent
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Abstract
The invention provides for multi-well plates with greater than 864 wells that comprise a layer of cycloolefin having low fluorescence and high transmittance. These multi-well plates are particularly well suited for fluorescence measurements.
Claims22
A multi-well plate for fluorescence measurements of biological and biochemical samples, comprising:
- more than 864 wells and each well comprising a wall and a bottom layer, said bottom layer with a low fluorescence and high transmittance portion comprising a cycloolefin copolymer, and
- a frame, wherein said wells are disposed in said frame and said wells can hold ten microliters or less in volume,
- wherein said multi-well plate is for detecting a fluorescence signal from a biological and biochemical sample and said multi-well plate has a footprint of a standard 96-well microtiter plate, and further wherein said low fluorescence and high transmittance portion is substantially all of said bottom layer.
A process for making a multi-well plate for fluorescence measurements of biological and biochemical samples with more than 864 wells and each well comprising a polymer wall, said process comprising:
- injection molding said multi-well plate with a bottom layer with low fluorescence and high transmittance, comprising a cycloolefin copolymer, and
- wherein said wells of said multi-well plate are disposed in a frame and said wells can hold ten microliters or less in volume, and said multi-well plate is for detecting a fluorescence signal from a biological or biochemical sample and said multi-well plate has a footprint of a standard 96-well microtiter plate, and further wherein said low fluorescence and high transmittance portion is substantially all of said bottom layer.
A kit, comprising:
- a) reagents for an assay,
- b) a multi-well plate for fluorescence measurements of biological and biochemical samples, comprising:
- i) more than 864 wells and each well comprising a wall and a bottom layer, said bottom layer with a low fluorescence and high transmittance portion comprising a cycloolefin copolymer, and
- ii) a frame, wherein said wells are disposed in said frame and said wells can hold ten microliters or less in volume;
- wherein said multi-well plate is for detecting a signal from a sample and said multi-well plate has a footprint of a standard 96-well microtiter plate, and further wherein said low fluorescence and high transmittance portion is substantially all of said bottom layer.
A multi-well plate for fluorescence measurements of biological and biochemical samples, comprsing:
- more than 864 wells in two dimensional linear arrays and each well comprising a wall and a bottom layer said bottom layer with a low fluorescence and high transmittance portion comprising a cycloolefin polymer, and
- a frame, wherein said wells are disposed in said frame, and said wells can hold ten microliters or less in volume,
- wherein said multi-well plate is for detecting a signal from a sample and said multi-well plate has a footprint of a standard 96-well microtiter plate, and further wherein said low fluorescence and high transmittance portion is substantially all of said bottom layer.
Dependencies are inferred from the claim text.