Protein from urine named component B
AssigneeApplied Research Systems ARS Holding N.V.
InventorAntonino Sirna
Life of this patent
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Abstract
A new protein is described obtainable from urine through an extraction and purification process by ion-exchange chromatography and high resolution chromatography.
Claims15
An isolated and purified polypeptide comprising the peptide sequence of SEQ ID NO:1 or its salts and functional derivatives selected from the group consisting of N-terminal or C-terminal acyl derivatives, or mixtures thereof.
An isolated DNA molecule comprising the nucleotide sequence of SEQ ID NO:
- 2.
An isolated cDNA molecule comprising the nucleotide sequence of SEQ ID NO:
- 3.
An essentially pure protein, comprising the amino acid sequence shown in SEQ ID NO:1, or its salts, obtainable through a process comprising the following steps:
- a) adsorption of urine, at pH acid, on kaolin and extraction with ammonia
- b) elution of fraction (a) on ion exchange resin with ammonia
- c) elution of fraction (b) on ion exchange resin with acetate buffer
- d) elution of fraction (c) on ion exchange resin with acetate buffer
- e) elution of fraction (d) on ion exchange resin with a mixture of acetate buffer and acetonitrile
- f) elution of fraction (e) ion exchange resin with acetate buffer
- g) elution of fraction (f) on ion exchange resin with acetate buffer
- h) elution of fraction (g) on ion exchange resin with a mixture of aqueous trifluoroacetic acid acetonitrile
- i) elution of fraction (h) on ion exchange resin with acetate buffer.
Dependencies are inferred from the claim text.