Chemically defined medium for human mesenchymal stem cells
AssigneeOsiris Therapeutics, Inc.
InventorsDaniel R. Marshak, James J. Holecek
Life of this patent
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Abstract
A composition and method for maintaining the viability of human mesenchymal precursor cells in a serum-free environment which composition includes (1) a minimum essential medium; (2) serum albumin; (3) an iron source; (4) insulin or an insulin-like growth factor; and (5) at least one amino acid selected from the group consisting of glutamine, arginine and and cysteine, and is free of serum. Also, a composition and method for culture expanding human mesenchymal precursor cells in a serum-free environment. This composition further includes a mitogen, paricularly a serotonergic agonist. The cells are preferably isolated human mesenchymal stem cells.
Claims12
A method for culturally expanding human mesenchymal stem cells comprising culturing an isolated homogenous population of viable human mesenchymal stem cells for a suitable time in a serum free medium which comprises (1) a minimum essential medium;
- (2) serum albumin;
- (3) an iron source;
- (4) insulin or an insulin-like growth factor;
- (5) glutamine;
- and (6) a mitogen selected from the group consisting of platelet derived growth factor and serotonin, which are present in amounts effective to culturally expand the human mesenchymal stem cells.
A composition which comprises an isolated homogenous population of viable human mesenchymal stem cells in a serum free medium comprising (1) a minimum essential medium;
- (2) serum albumin;
- (3) an iron source;
- (4) insulin or an insulin-like growth factor;
- (5) glutamine;
- and (6) a mitogen selected from the group consisting of platelet derived growth factor and serotonin which are present in amounts effective to culturally expand the human mesenchymal stem cells in the absence of serum.
Dependencies are inferred from the claim text.