# US 5,908,773 A · KSHV positive cell lines

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- Number: US 5,908,773 A
- Document ID: US5908773A
- Status: Granted
- Kind: A
- Application number: 08/717,291
- Filed: 1996-09-20
- Granted: 1999-06-01
- Earliest claimed: 1996-09-20
- Assignees: Cornell Research Foundation, Inc.
- Inventors: Ethel Cesarman; Leandros Arvanitakis; Daniel M. Knowles; Enrique Mesri
- Web page: https://patentgrep.com/patent/US5908773A

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 2710/16451: Methods of production or purification of viral material
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The invention provides a cell line comprising Kaposi's sarcoma-associated herpesvirus (KSHV). Preferably, the cell line is a body cavity-based lymphoma cell line, and more preferably, the cell line does not harbor Epstein-Barr virus. Two cell lines designated BC-2 and BC-3 are thus provided. These cell lines can be used in a method of propagating KSHV. The method comprises culturing the cell line, wherein the KSHV within the cell line thereby propagates. The BC-3 cell line, in particular, can be used in a method of propagating KSHV in the absence of Epstein-Barr virus. The method comprises culturing the cell line designated BC-3, wherein the KSHV within the cell line thereby propagates. Further provided is a purified viral suspension of KSHV, as well as a composition comprising purified KSHV and a suitable carrier.

## Claims

### Claim 1

A cell line comprising Kaposi's sarcoma-associated herpesvirus, wherein the cell line is designated BC-2 and is deposited with the ATCC as Accession No. CRL 2231.

### Claim 2

A cell line comprising Kaposi's sarcoma-associated herpesvirus, wherein an Epstein-Barr virus is not present in the cell line and wherein the cell line is designated BC-3 and is deposited with the ATCC as Accession No. CRL 2277.

### Claim 3 (depends on 1)

A method of propagating Kaposi's sarcoma-associated herpesvirus, said method comprising:
culturing the cell line of claim 1 under conditions effective to propagate said Kaposi's sarcoma-associated herpesvirus within said cell line.

### Claim 4 (depends on 2)

A method of propagating Kaposi's sarcoma-associated herpesvirus in the absence of Epstein-Barr virus, said method comprising:
culturing the cell line of claim 2 under conditions effective to propagate said Kaposi's sarcoma-associated herpesvirus within said cell line.