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US 5,908,772 AGranted
Gene encoding lacto-N-biosidase
AssigneeTakara Shuzo Co., Ltd.
InventorsMasanori Mitta, Mutsumi Sano, Ikunoshin Kato
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Abstract
An isolated DNA having a sequence encoding a polypeptide possessing lacto-N-biosidase activity or functionally equivalent variants thereof; and a method for producing a polypeptide possessing lacto-N-biosidase activity or functionally equivalent variants thereof using the isolated DNA by recombinant DNA technology.
Claims15
An isolated DNA encoding a polypeptide possessing lacto-N-biosidase activity, the isolated DNA being selected from the group consisting of:
- (a) a DNA having a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1;
- (b) a DNA having the nucleotide sequence of SEQ ID NO:2;
- and
- (c) a DNA which hybridizes to (b) above at 65.degree. C. for 20 hours in a solution containing 6.times. SSC, 1% sodium lauryl sulfate, 100 .mu.g/ml salmon sperm DNA and 5.times. Denhardt's solution.
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Dependencies are inferred from the claim text.