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US 5,906,925 AGranted
Microorganisms and methods for overproduction of DAHP by cloned pps gene
OwnerJames C. Liaoinventor
Life of this patent
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Abstract
Genetic elements comprising expression vectors and a gene coding for phosphoenol pyruvate synthase is utilized to enhance diversion of carbon resources into the common aromatic pathway and pathways branching therefrom. The overexpression of phosphoenol pyruvate synthase increases DAHP production to near theoretical yields.
Claims28
A method of producing 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) comprising the steps of:
- (a) transforming a microorganism with genetic elements comprising pps and tkt genes wherein said genes are expressed when said microorganism is grown in minimal medium containing glucose;
- (b) overexpressing the pps and tkt genes in said microorganisms incubated in minimal medium containing glucose;
- and
- (c) recovering DAHP from said media.
1 more element
A method of producing tryptophan comprising the steps of:
- (a) transforming a microorganism with genetic elements comprising pps, tkt, and serA genes wherein said genes are expressed when said microorganism is grown in a minimal medium containing glucose;
- (b) overexpressing the pps, tkt, and serA genes in said microorganisms incubated in the media minimal medium containing glucose;
- and
- (c) recovering tryptophan from said media.
1 more element
A method of producing phenylalanine comprising the steps of:
- (a) transforming a microorganism with genetic elements comprising pps, tkt, and pheA.sup.fbr genes wherein said genes are expressed when said microorganism is grown in minimal medium containing glucose;
- (b) overexpressing the pps, tkt, and pheA.sup.fbr genese in said microorganisms incubated in minimal medium containing glucose;
- and
- (c) recovering phenylalanine from said media.
1 more element
A method of producing quinic acid comprising the steps of:
- (a) transforming a microorganism with genetic elements comprising pps, tkt, and qad genes wherein said genes are expressed when said microorganism is grown in minimal medium containing glucose;
- (b) overexpressing the pps, tkt, and qad genes in said microorganisms incubated in minimal medium containing glucose;
- and
- (c) recovering quinic acid from said media.
1 more element
Dependencies are inferred from the claim text.