Production of human recombinant collagen in the milk of transgenic animals
AssigneesCohesion Technologies, Inc., Pharming Holding NV
InventorRichard A. Berg
Life of this patent
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Abstract
Production of human procollagen or collagen in cells which ordinarily do not produce these molecules is effected by constructing expression systems compatible with mammary glands of non-human mammals. For example, expression systems can be microinjected into fertilized oocytes and reimplanted in foster mothers and carried to term in order to obtain transgenic non-human mammals capable of producing milk containing recombinant human procollagen or collagen. Human procollagen or collagen produced in this manner can be made of a single collagen type uncontaminated by other human or non-human collagens.
Claims6
A method of preparing a single type of trimeric human procollagen, collagen, or a mixture of such procollagen and collagen, which method comprises the step of:
- recovering said procollagen, collagen, or mixture from milk of a transgenic mammal comprising an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete said procollagen, collagen, or mixture comprising said chain in said milk without extracellular aggregation of said procollagen, collagen or mixture which would prevent excretion from said mammary gland.
A transgenic nonhuman mammal whose somatic and germ cells contain an expression system comprising a coding nucleotide sequence encoding a human procollagen polypeptide chain operably linked to a control nucleotide sequence that effects expression specifically in milk protein-secreting epithelial cells of a mammary gland in said mammal, and said cells express said coding nucleotide sequence to produce said polypeptide chain, and secrete a single type of human procollagen, collagen, or a mixture thereof into the milk of said mammal, wherein said milk comprises trimeric collagen, procollagen, or a mixture thereof, in recoverable amounts without extracellular aggregation which would prevent excretion from said mammary gland.
Dependencies are inferred from the claim text.