US 5,891,722 A · Antibodies to free IGFBP-1

Abstract

Antibodies having binding affinity for free IGFBP-1, biological compositions including antibodies having binding affinity for free IGFBP-1, kits for detecting free IGFBP-1 using the antibodies, and cell lines for producing the antibodies are provided. Also provided are devices and methods for detecting free IGFBP-1 and a rupture in a fetal membrane based on the presence of amniotic fluid in a vaginal secretion, as indicated by the presence of free IGFBP-1 in the vaginal secretion. The antibodies that are provided may be characterized by their ability to selectively recognize those IGFBP-1 molecules which are free of IGF-1 and IGF-2, i.e., antibodies which have a binding affinity for free IGFBP-1 that is greater than a binding affinity of the antibody to bound IGFBP-1. These antibodies may also be characterized by their competition with IGF-1 and IGF-2 for binding to IGFBP-1.

Claims11

Dependent claims

A pair of monoclonal antibodies for use in detecting insulin-like growth factor binding protein-1 (IGFBP-1) uncompleted to insulin-like growth factor-1 (IFG-1) and insulin-like growth factor-2 (IGF-2) (free IGFBP-1), comprising:

a first anti-IGFBP-1 monoclonal antibody whose binding to said free IFGBP-1 is inhibited in the presence of said IGF-1 or said IGF-2, and
a second anti-IGFBP-1 monoclonal antibody whose binding to said free IGFBP-1 is inhibited in the presence of said IGF-1 or said IGF-2,
where said first and second monoclonal antibodies are non-cross reactive and said first anti-IGFBP-1 monoclonal antibody is Mroduced by a cell line identified by ATCC designation HB-12279 and said second anti-IGFBP-1 monoclonal antibody is produced by a cell line identified by ATCC designation HB-12280.

Dependencies are inferred from the claim text.

Antibodies to free IGFBP-1

Life of this patent

Antibodies to free IGFBP-1

Life of this patent

Abstract

Claims11