Method for inactivating a virus
AssigneePentose Pharmaceuticals, Inc.
InventorsEdward I. Budowsky, Samuel K. Ackerman
Life of this patent
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Abstract
The present invention concerns a method for inactivating a virus for use in e.g. a vaccine, by treating the virus with an ethyleneimine at a pH of less than 7.0, and methods of treatment with the inactivated virus.
Claims8
A method for inactivating a virus selected from the group consisting of polio virus, rabies virus, yellow fever virus, Japanese encephalitis virus, tick-borne encephalitis virus, measles virus, mumps virus, Ross River virus, rotavirus, and rubella, said method comprising treating said virus with an inactivating amount of a composition comprising an ethyleneimine at a pH less than 7.0.
A method for inactivating a virus selected from the group consisting of poliovirus, rabies virus, yellow fever virus, Japanese encephalitis virus, tick-borne encephalitis virus, measles virus, mumps virus, Ross River virus, rotavirus, and rubella, said method comprising contacting said virus with monomeric ethyleneimine at a pH less than 7.0 which has been determined by
- (a) treating a plurality of samples of said virus with an inactivating amount of monomeric ethyleneimine at a plurality of different pH values;
- (b) measuring in each treated virus sample a characteristic selected from viral inactivation and immunogenicity;
- and
- (c) selecting the pH which provides, relative to the other pH values of step (a), a higher viral inactivation or a higher immunogenicity, or a combination thereof.
Dependencies are inferred from the claim text.