Mutated penicillin G acylase genes
AssigneeGist-Hrocades
InventorsJan M. Van Der Laan, Adriana M. Riemens, Wilhelmus J. Quax
Life of this patent
Estimating the term…
Abstract
New mutant .beta.-lactam Penicillin G acylases are provided, exhibiting altered substrate specificities. These Penicillin G acylases are obtained by expression of a gene encoding for said Penicillin G acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type Penicillin G acylase.
Claims12
An isolated mutant prokaryotic Penicillin G acylase or its preenzmye or preproenzyme having:
- an amino acid substitution at one or more of the positions corresponding to A139, A140, A142 and A148 to A152 as set forth in SEQ ID NO:
- 27, and B20 to B27, B31, B49 to B52, B56, B57, B65, B67 to B72, B154 to B157, B173 to B179, B239 to B241, B250 to B263, B379 to B387, B390, B455, and B474 to B480 as set forth in SEQ ID NO:
- 32 in Alcaligenes faecalis Penicillin G acylase or its pre- or preproenzyme;
- and an altered substrate specificity or altered specific activity relative to the corresponding wild-type unsubstituted Penicillin G acylase.
A nucleic acid sequence encoding a mutant acylase as defined in any one of the preceding claims.
An isolated mutant prokaryotic Penicillin G acylase or its preenzyme or preproenzyme having an amino acid substitution at a position corresponding to A143 (Met) as set forth in SEQ ID NO:27 wherein A143 (Met) is changed to Arg, Lys, Cys, Gly, Thr, or Asp.
Dependencies are inferred from the claim text.