Peptide synthesis and purification by fusion to penI protein or precipitation effective portion thereof
AssigneeDade International Inc.
InventorsRony Tal, Hing C. Wong, Clayton Casipit, Pierre-Andre Chavaillaz, Vaughan Wittman
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Abstract
The present invention provides novel methods for the synthesis and isolation and purification of a peptide of interest (target peptide). In particular, the invention relates to peptide synthesis, isolation and purification methods that comprise use of penI fusion polypeptides and related gene fusion constructs that encode such polypeptides.
Claims38
A method for producing a target peptide, comprising:
- (a) providing in solution a fusion polypeptide that comprises a target peptide and a penI protein or precipitation effective portion thereof;
- and
- (b) contacting said polypeptide with an aqueous solution having an ionic strength sufficient to precipitate the fusion polypeptide.
A DNA expression vector capable of expressing a penI protein or precipitation effective potion thereof fused to a target peptide, comprising:
- a DNA sequence coding for the penI protein or precipitation effective portion thereof;
- and
- a DNA sequence coding for the target peptide.
A fusion polypeptide comprising a penI protein or precipitation effective portion thereof fused to a target peptide.
A method of immunization of a mammal comprising administering to the mammal a fusion polypeptide that comprises a penI protein or portion thereof fused to a target peptide whereby said administration elicits a desired immune response by the mammal.
A method for the selection of a target polypeptide comprising:
- transforming host cells with expression vectors that each contains a gene construct that codes for a fusion polypeptide comprising a target peptide and a penI protein or precipitation effective portion thereof;
- culturing the transformed host cells under conditions suitable for expression of the fusion polypeptide;
- and
- selecting host cells that express the target polypeptide.
Dependencies are inferred from the claim text.