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US 5,886,244 AGranted
Stable transformation of plant cells
AssigneePioneer Hi-Bred International, Inc.
InventorsDwight T. Tomes, Arthur Weissinger, John C. Sanford, Theodore M. Klein
Life of this patent
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Abstract
Fertile, stably transformed Zea mays plants can be produced by introducing agronomic trait-encoding DNA, via microprojectile bombardment, into maize embryonic callus or suspension culture that has been broken into clumps having a maximum diameter of about 650 microns, where the bombardment is effected within six days of the formation of the clumps.
Claims11
A method for producing a fertile and stably transformed Zea mays plant, comprising the steps of:
- (a) providing foreign DNA that comprises an expression vector carrying a gene that encodes an agronomic trait;
- (b) providing maize embryonic callus or suspension culture that has been broken into clumps having a maximum diameter of about 650 microns;
- then
- (c) introducing said foreign DNA into said callus or suspension culture by at least one microparticle bombardment, wherein said bombardment is effected within 0 to 6 days of formation of said clumps;
- and then
- (d) regenerating a fertile, stably transformed Zea mays plant from said callus or suspension culture.
3 more elements
Dependencies are inferred from the claim text.