Engineering oral tissues
AssigneeThe Regents of the University of Michigan
InventorsDavid J. Mooney, Robert B. Rutherford
Life of this patent
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Abstract
Disclosed are methods for regenerating dental and oral tissues from viable cells using ex vivo culture on a structural matrix. The regenerated oral tissues and tissue-matrix preparations thus provided have both clinical applications in dentistry and oral medicine and are also useful in in vitro toxicity and biocompatibility testing.
Claims109
A method for culturing oral tissue cells, comprising growing viable oral tissue cells on a synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow proliferation of viable oral tissue cells.
A method for generating a tissue sample comprising oral tissue cells, the method comprising culturing viable starting cells obtained from an oral tissue sample on a synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow the formation of a tissue sample that comprises viable oral tissue cells.
A method for culturing oral tissue cells, comprising growing viable starting cells obtained from an oral tissue sample in functional association with a three dimensional synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow proliferation and organization of viable oral tissue cells to form a three dimensional biological structure comprising cells that express at least one marker indicative of oral tissue cells.
A method for generating an oral tissue sample, comprising growing viable starting cells obtained from an oral tissue sample in functional association with a three dimensional synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow proliferation and organization of viable oral tissue cells to form a three dimensional oral tissue structure comprising a population of cells that express at least one biological marker indicative of oral tissue cells.
A method for reconstructing an oral tissue, comprising obtaining a composition comprising viable cells from an oral tissue of an animal, culturing the viable cells of said composition on a synthetic matrix ex vivo under conditions effective and for a period of time sufficient to allow the formation of the corresponding native oral tissue.
A method for filling a root canal, comprising culturing viable starting cells on a synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow the formation of regenerated dental pulp tissue within said synthetic matrix and implanting said regenerated dental pulp tissue into the root canal.
A method for delivering a selected factor to an animal, comprising culturing viable oral tissue cells on a synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow formation of a tissue sample that comprises viable oral tissue cells and delivering said tissue sample to an oral tissue site of an animal in the presence of a selected factor.
A population of cultured oral tissue cells prepared by a process comprising culturing viable starting cells obtained from an oral tissue sample on a synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow proliferation of viable oral tissue cells.
A tissue sample that comprises viable oral tissue cells prepared by a process comprising culturing viable starting cells obtained from an oral tissue sample on a synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow formation of a tissue sample that comprises viable oral tissue cells.
A three dimensional synthetic matrix housing a regenerated oral tissue sample prepared by a process comprising culturing viable starting cells obtained from an oral tissue sample on a synthetic matrix in vitro under conditions effective and for a period of time sufficient to allow proliferation and organization of said cells to form an oral tissue sample within said synthetic matrix.
A method for testing the suitability of a candidate substance for use in the oral cavity, comprising applying a candidate substance to a regenerated, three-dimensional oral tissue sample, and analyzing the effect of said candidate substance on the viability of said tissue sample, wherein a candidate substance that is not unacceptably toxic to said tissue sample is a substance suitable for use in the oral cavity.
An oral or dental kit comprising, in a container means, a three dimensional synthetic matrix housing a regenerated oral tissue sample.
Dependencies are inferred from the claim text.