Enzyme with xylanase activity
AssigneeNovo Nordisk A/S
InventorsLene Venke Kofod, Markus Sakari Kauppinen, Stephan Christgau, Hans Peter Heldt-Hansen, Henrik Dalb.o slashed.ge, Lene Nonboe Andersen, Joan Qi Si, Tina Sejersg.ang.rd Jacobsen, Niels Munk, Anette Mullertz
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Abstract
An enzyme exhibiting xylanase activity, which enzyme is immunologically reactive with an antibody raised against a purified xylanase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be used for degrading plant cell wall components e.g. in the preparation of feed, in baking, in the paper and pulp industry and in connection with separation of wheat into starch and gluten.
Claims44
A DNA construct which comprises a DNA sequence encoding an enzyme having xylanase activity as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-birch xylan, and which DNA sequence hybridizes to a DNA depicted in SEQ ID NO. 5 under the following conditions:
- hybridizing in 5.times. SSC, 5.times. Denhardt's solution, 0.5% SDS and 100 .mu.g/ml salmon sperm DNA for 16 hrs. at about 65.degree. C. followed by washes in 5.times. SSC at 65.degree. C., 2.times. SSC, 0.5% SDS, 0.2.times. SSC, 0.5% SDS and 5.times. SSC.
A DNA construct which comprises a DNA sequence encoding an enzyme having sylanase activity depicted in SEQ ID NO:6 or mutant thereof having the same xylanase activity as the xylanase depicted in SEQ ID NO. 6 as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-birch xylan, said said mutant also having a pH optimum of 5-6 and a temperature optimum of 40.degree.-50.degree. C.
A DNA construct which comprises a DNA sequence derived from Aspergillus aculeatus encoding an enzyme having xylanase activity as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-birch xylan, said enzyme having a pH optimum of 4-5 and at a temperature range of 70.degree.-80.degree. C. and in which said enzyme is encoded by a DNA sequence comprising the partial DNA sequence depicted in SEQ ID NO. 41.
A DNA construct which comprises a DNA sequence derived from Aspergillus aculeatus encoding an enzyme having xylanase activity as measured by release of reducing sugars from birch xylan or by release of blue color from AZCL-birch xylan, said enzyme having a pH optimum of 5-6 and at a temperature range of 40.degree.-50.degree. C. and in which said enzyme is encoded by a DNA sequence comprising the partial DNA sequence depicted in SEQ ID NO. 42.
Dependencies are inferred from the claim text.