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AssigneeSoane Biosciences, Inc.
InventorsHerbert H. Hooper, Alexander P. Sassi, David S. Soane, Young Bae
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Electrophoretic media are provided comprising at least un-crosslinked polymers which have a temperature reversible transition from low viscosity to high viscosity, so as to be pourable at one temperature, while providing sieving properties at another temperature. Optionally included with the un-crosslinked polymers having a reversible temperature responsive viscosity change are gelling agents and polymers which do not have the reversible temperature responsive viscosity change. The subject compositions have excellent clarity, provide excellent separation and resolution, handling properties, and mechanical strength. The compositions are compatible with transfer of separated components from the gel to an accepting membrane.
An electrophoretic device comprising an electrophoretic gel holder containing an electrophoretic medium in a capillary or microchannel, said medium comprising a component consisting essentially of an un-crosslinked thermoreversible acrylamide copolymer containing a derivatized acrylamide in an amount sufficient to provide in said medium over a range of temperatures less than 20.degree. C. a transition between a first state and a second state wherein said medium has a viscosity in said second state at least 100% higher than in said first state.
An electrophoretic device comprising an electrophoretic gel holder containing an electrophoretic medium, said medium comprising an un-crosslinked thermoreversible derivatized cellulose in an amount sufficient to provide in said medium over a range of temperatures less than 20.degree. C. a transition between a first state and a second state wherein said medium has a viscosity in said second state at least 100% higher than in said first state.
A method for preparing an electrophoretic device for electrophoretic separation of a sample, comprising steps of:
Dependencies are inferred from the claim text.