Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
AssigneeAclara Biosciences, Inc.
InventorsAlexander P. Sassi, Shi Lin, M. Goretty Alonso-Amigo, Herbert H. Hooper
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Abstract
Thermoreversible hydrogels comprising non-ionic, uncrosslinked copolymers, and methods of their use in electrophoresis, are provided. The subject copolymers comprise polyacrylamide backbones, where a portion of the acrylamide monomeric units comprise hydrogen bonding groups as N-substituents. Combination of the subject copolymers with an aqueous phase provides thermoreversible hydrogels which find use as separation media in electrophoretic applications.
Claims14
A copolymer of acrylylglycinamide and acrylamide, wherein the weight percent ratio of acrylylglycinamide to acrylamide of said copolymer is in the range from about 15:85 to about 99:1.
A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
- a non-ionic, uncrosslinked copolymer comprising first and second monomeric units in a weight percent ratio of from about 15:85 to about 99:1, wherein said first monomeric unit is acrylylglycinamide;
- and said second monomeric unit is an acrylamide.
- a continuous liquid phase acrylylglycinamide;
- and said second monomeric unit is an acrylamide.
A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
- a polyacrylamide backbone, wherein a portion of the acrylamide monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding, wherein said hydrogen bonding N-substituent groups comprise a carbamyl group;
- an additional, non-proteinaceous uncrosslinked polymer;
- and
- an aqueous phase.
A non-ionic, uncrosslinked copolymer capable of forming a thermoreversible hydrogel when combined with an aqueous phase, said copolymer comprising a polyacrylamide backbone wherein said copolymer comprises first and second monomeric units in a ratio between about 15:85 and 99:1 by weight, and wherein said first monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding, said N-substituent hydrogen bonding groups comprising a heterocyclic nitrogen base, and said second monomeric unit is an acrylamide.
A non-ionic, uncrosslinked copolymer capable of forming a thermoreversible hydrogel when combined with an aqueous phase, said copolymer comprising first and second monomeric units in a weight percent ratio of from about 15:85 to about 99:1, wherein
- said first monomeric unit is of the formula:
- ##STR4## wherein:
- X is H or CH.sub.3,
- Y is a bond or a linking group of from 1 to 6 carbon atoms;
- and
- Z is a group comprising a hydrogen bonding moiety, wherein said hydrogen bonding moiety comprises a heterocyclic nitrogen base;
- and
- said second monomeric unit is an acrylamide.
A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
- a non-ionic, uncrosslinked copolymer comprising a polyacrylamide backbone, wherein about 15 to 99 weight percent of the acrylamide monomeric units of said copolymer comprise N-substituent groups capable of hydrogen bonding, wherein said N-substituent groups comprise a heterocyclic nitrogen base;
- and
- a continuous fluid phase.
A thermoreversible hydrogel suitable for use as a separation medium for electrophoresis, said hydrogel comprising:
- a non-ionic, uncrosslinked copolymer comprising first and second monomeric units in a weight percent ratio of from about 15:85 to about 99:1, wherein said first monomeric unit is of the formula:
- ##STR5## wherein:
- X is H or CH.sub.3,
- Y is a bond or a linking group of from 1 to 6 carbon atoms;
- and
- Z is a group comprising a hydrogen bonding moiety, wherein said hydrogen bonding moiety comprises a heterocyclic nitrogen base;
- and
- said second monomeric unit is an acrylamide;
- and
- a continuous liquid phase.
Dependencies are inferred from the claim text.