Biomarkers and targets for diagnosis, prognosis and management of prostate disease
AssigneeUrocor, Inc.
InventorsGang An, S. Mark O'Hara, David Ralph, Robert Veltri
Life of this patent
Estimating the term…
Abstract
Disclosed are diagnostic techniques for the detection of human prostate cancer. Genetic probes and methods useful in monitoring the progression and diagnosis of prostate cancer are described. The invention relates particularly to probes and methods for evaluating the presence of RNA species that are differentially expressed in prostate cancer compared to normal human prostate or benign prostatic hyperplasia.
Claims64
A method for detecting prostate cancer or benign prostatic hyperplasia cells in a biological sample comprising:
- a) providing nucleic acids from said sample;
- b) amplifying said nucleic acids to form nucleic acid amplification products;
- c) contacting said nucleic acid amplification products with an oligonucleotide probe that will hybridize under stringent conditions with an isolated nucleic acid having a sequence selected from a group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:45 and SEQ ID NO:46;
- d) detecting the nucleic acid amplification products which hybridize with said probe;
- and
- e) quantifying the amount of said nucleic acid amplification products that hybridize with said probe.
A method for detecting prostate cancer or benign prostatic hyperplasia cells in a biological sample comprising:
- a) providing nucleic acids from said sample;
- b) providing primers that will selectively amplify an isolated nucleic acid having a sequence selected from a group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:45 and SEQ ID NO:46;
- c) amplifying said nucleic acids with said primers to form nucleic acid amplification products;
- d) detecting said nucleic acid amplification products;
- and
- e) quantifying the amounts of said nucleic acid amplification products formed.
Dependencies are inferred from the claim text.