Synthetic plant genes from BT kurstaki and method for preparation
AssigneeMonsanto Company
InventorsDavid A. Fischhoff, Frederick J. Perlak
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Abstract
A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, and B.t.k. HD-73.
Claims8
A heterologous gene construct comprising a structural coding sequence which encodes an insecticidal protein derived from B.k. HD-1, said structural coding sequence comprising SEQ ID NO:20.
A heterologous gene construct comprising a structural coding sequence which encodes an insecticidal protein derived from B.t.k. HD-73, said structural coding sequence comprising SEQ ID NO:21.
A heterologous gene construct comprising a structural coding sequence which encodes an insecticidal protein derived from B.t.k. HD-1, said structural coding sequence comprising SEQ ID NO:22.
A heterologous gene construct comprising a structural coding sequence which encodes an insecticidal protein derived from B.t.k. HD-73, said structural coding sequence comprising SEQ ID NO:23.
A heterologous gene construct comprising a structural coding sequence which encodes an insecticidal protein derived from B.t.k. HD-73, said structural coding sequence comprising SEQ ID NO:24.
A heterologous gene construct comprising a structural coding sequence which encodes an insecticidal protein derived from B.t.k. HD-73, said structural coding sequence comprising SEQ ID NO:25.
A heterologous gene construct comprising a structural coding sequence which encodes an insecticidal protein derived from B.t.k. HD-73, said structural coding sequence comprising SEQ ID NO:26.
A heterologous gene construct comprising a structural coding sequence which encodes a fusion protein comprising the N-terminal 610 amino acids of a toxin protein derived from B.t.k. HD-1 and the C-terminal 567 amino acids of a toxin protein derived from B.t.k. HD-73, said structural coding sequence comprising SEQ ID NO:27.
Dependencies are inferred from the claim text.