Process for fractionating polyethylene glycol (PEG)-protein adducts and an adduct of PEG and granulocyte-macrophage colony stimulating factor
AssigneePolyMASC Pharmaceuticals plc
InventorsCristina Delgado, Gillian Elizabeth Francis, Derek Fisher
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Abstract
The invention relates to fractionating a mixture of polyethylene glycol (PEG)-protein adducts having different degrees of PEG substitution by partitioning the PEG-protein adducts in a PEG-containing aqueous biphasic system according to the degree of PEG substitution. A new PEG-gm-CSF obtained by the process is useful in pharmaceutical compositions for use in therapeutic or diagnostic methods.
Claims8
A process for producing a biologically-active PEG-protein adduct comprising reacting a 2,2,2-trifluoreothane sulphonyl derivative of a PEG having a single derivatizable hydroxyl group with a recombinantly produced protein selected from the group consisting of an antibody, asparaginase, glutaminase-asparaginase, uricase, arginase superoxide dismutase, lactoferrin, streptokinase, plasma-streptokinase complex, adenosine deaminase, a peptide hormone, a mast cell gowth factor, G-CSF and GM-CSF.
A process for producing a biologically-active PEG-protein adduct comprising reacting a 2,2,2-tdfluoroethane sulphonyl monomethoxy-PEG with a recombinantly produced protein selected from the group consisting of an antibody, asparaginase, glutaminiase-asparaginase, uricase, arginase, superoxide dismutase, lactoferrin, streptokinase, plasma-streptokinase complex, adenosine deaminase, a peptide hormone, a mast cell growth factor, G-CSF and GM-CSF.
Dependencies are inferred from the claim text.