US 5,879,903 A · Phosphinothricin-resistance gene, and its use

Abstract

Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.

Claims6

Dependent claims

A method of using a phosphinothricin (PTC)-resistance gene as a resistance marker in bacteria comprising the steps of transforming the bacteria with the phosphinothricin (PTC)-resistance gene,

culturing the bacteria in a medium,
exposing the bacteria to phosphinothricin, and
determining whether the bacteria is resistant to the phosphinothricin, wherein the phosphinothricin (PTC)-resistance gene is obtainable by selecting Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT), cutting with BamHI the total DNA from the resistant strains, cloning a fragment 4.0 kb in size, and selecting for PTT resistance.

Dependencies are inferred from the claim text.

Facts

Application

08/479,717Filed 7 Jun 1995 · continuation

Priority

DE 36 28 747.423 Aug 1986

Granted

9 Mar 1999

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12P 41/007
By reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation…
C12N 9/1029
Acyltransferases: transferring groups other than amino-acyl groups
C12N 15/64
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: general methods for preparing…
C12N 15/8209
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…

Family

Serial605,131Parent of this continuation · filed 31 Oct 1990

Serial795,275Parent of this division · filed 20 Nov 1991

Serial115,651Parent of this continuation · filed 3 Sep 1993

Serial88,118Parent of this continuation · filed 21 Aug 1997

US 5,273,894 AParent of this division

US 5,879,903 AThis patent

From the US filing history. Foreign family members aren’t listed.

Phosphinothricin-resistance gene, and its use

Life of this patent

Phosphinothricin-resistance gene, and its use

Life of this patent

Abstract

Claims6