Production of antibodies
AssigneeGlaxo Wellcome Inc.
InventorsJames Scott Crowe, Alan Peter Lewis
Life of this patent
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Abstract
This invention relates to the preparation of recombinant primate antibodies by DNA technology; Micro-RNA techniques for production of the same; recombinant non-human primate antibodies; formulations containing the same; the use of such antibodies in the prophylaxis and treatment of humans, and diagnostic uses of such antibodies.
Claims14
A process for the production of a recombinant primate antibody comprising:
- (i) selecting a primate lymphocyte-derived cell line that is capable of expressing a desired antibody;
- (ii) isolating RNA from the cell line and separating mRNA from the other RNAs so isolated;
- (iii) synthesising cDNA from the mRNA and inserting the cDNA into a cloning vector;
- (iv) transforming a host cell with the vector containing the cDNA to obtain a library;
- (v) screening the library for cDNA encoding the entire constant and variable regions of the heavy and light chains;
- (vi) inserting the cDNA encoding the heavy and light chains into an expression vector;
- (vii) transfecting a host cell with the expression vector containing the cDNA;
- and
- (viii) culturing the transfected host cell and isolating the desired antibody.
A process for the production of a recombinant antibody comprising:
- i) micro-RNA preparation from approximately 1000 cells;
- ii) generation of a size-selected cDNA library;
- iii) screening the library for cDNA encoding the entire constant and variable regions of the antibody and light chains and isolating the same;
- iv) inserting the cDNA encoding the heavy and light chains into an expression vector;
- v) transfecting a host cell with the expression vector containing the cDNA;
- and
- vi) culturing the transfected host cell and isolating the desired antibody.
A vector suitable for transfection of a host cell comprising cDNA encoding primate antibody heavy and light chains.
A eukaryotic cell-line transfected with cDNA for the expression of primate antibody heavy and light chains.
A process for the expression of cDNA encoding primate antibody heavy and light chains, comprising transfecting a eukaryotic host cell with a vector or vectors suitable for the expression of said cDNA.
Dependencies are inferred from the claim text.