Hapten-carrier conjugates for use in drug-abuse therapy and methods for preparation of same

A nicotine hapten-carrier conjugate comprising the structure shown in FIG. 17b, wherein A, B, C, D, E and F are side chains of nicotine, which are independently selected from the group consisting of chemical moieties, said chemical moieties are identified by CJ reference number:

• ______________________________________ CJ 0 Q CJ 1 (CH.sub.2).sub.n Q CJ 1.1 CO.sub.2 Q CJ 1.2 COQ CJ 1.3 OCH.sub.3 CJ 2 OCO(CH.sub.2).sub.n Q CJ 2.1 OCOCH.dbd.Q CJ 2.2 OCOCH(O)CH.sub.2 CJ 2.3 OCO(CH.sub.2).sub.n CH(O)CH.sub.2 CJ 3 CO(CH.sub.2)nCOQ CJ 3.1 CO(CH.sub.2).sub.n CNQ CJ 4 OCO(CH.sub.2).sub.n COQ CJ 4.1 OCO(CH.sub.2).sub.n CNQ CJ 5 CH.sub.2 OCO(CH.sub.2).sub.n COQ CJ 5.1 CH.sub.2 OCO(CH.sub.2).sub.n CNQ CJ 6 CONH(CH.sub.2).sub.n Q CJ 7 Y(CH.sub.2).sub.n Q CJ 7.1 CH.sub.2 Y(CH.sub.2).sub.n Q CJ 8 OCOCH(OH)CH.sub.2 Q CJ 8.1 OCO(CH.sub.2).sub.n CH(OH)CH.sub.2 Q CJ 9 OCOC.sub.6 H.sub.5 CJ 10 See FIG. 2b CJ 11 YCO(CH.sub.2).sub.n COQ;
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• wherein Y is sulfur (S), oxygen (O), or an amine (NH), wherein n is an integer from 3 to 20, and wherein Q is selected from the group consisting of:
• H, OH, OCH3, CH2, CH3, COOH, halogens, activated esters, acyl halides, acyl azides, alkyl halides, N-maleimides, imino esters, isocyarlate, isothiocyanate, and a T cell epitope-containing carriers;
• with the proviso that Q in at least one of A, B, C, D, E, or F comprises a carrier containing at least one T cell epitope said carrier selected from the group consisting of cholera toxin B (CTB), diphtheria toxin, tetanus toxoid, pertussis toxin, ricin B subunit, retrovirus nucleoprotein (retro NP), rabies ribonucleoprotein (Rabies RNP), Tobacco Mosaic Virus, and vesicular stomatitis vius nudeocapsid protein (VSV-N).