CDR-grafted type III anti-CEA humanized mouse monoclonal antibodies
AssigneeImmunomedics, Inc.
InventorsHans J. Hansen, Kathryn L. Armour
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Abstract
A humanized monoclonal antibody, comprising the complementarity-determining regions of a parental murine Class III, anti-CEA monoclonal antibody engrafted to the framework regions of a heterologous antibody, wherein the humanized monoclonal antibody retains the binding specificity of, but is less immunogenic in a heterologous host than, the parental murine monoclonal antibody A preferred murine Class III, anti-CEA monoclonal antibody is the MN-14 antibody and the preferred heterologous antibody is from a human. Also provided are DNA constructs and vectors for producing the humanized monoclonal antibodies, and diagnostic and therapeutic conjugates using same.
Claims30
A humanized monoclonal antibody, comprising the complementarity-determining regions (CDRs) of a parental murine Class III, anti-CEA MN-14 monoclonal antibody, wherein said humanized antibody retains the binding specificity of said MN-14 monoclonal antibody but is less immunogenic in a human subject than is said parental murine MN-14 monoclonal antibody, and wherein said humanized monoclonal antibody heavy and light chain variable regions are selected from the group consisting of KLHuVHAIG/HuVK (SEQ. ID NO. 13/SEQ. ID NO. 19), KLHuVHAIGA/HuVK (SEQ. ID NO. 14/SEQ. ID NO. 19) and KLHuVHAIGAY/HuVK (SEQ. ID NO. 15/SEQ. ID NO. 19).
A humanized MN-14 heavy chain comprising the heavy chain variable region KLHuVHAIG (SEQ. ID NO. 13).
A humanized MN-14 heavy chain comprising the heavy chain variable region KLHuVHAIGA (SEQ. ID NO. 14).
A humanized MN-14 heavy chain comprising the heavy chain variable region KLHuVHAIGAY (SEQ. ID NO. 15).
A humanized MN-14 light chain comprising the light chain variable region HuVK (SEQ. ID NO. 19).
An expression vector containing a polynucleotide encoding a humanized MN-14 heavy chain comprising a heavy chain variable region selected from the group consisting of KLHuVHAIG (SEQ. ID NO. 13), KLHuVHAIGA (SEQ.ID NO. 14) and KLHuVHAIGAY (SEQ. ID NO. 15).
Dependencies are inferred from the claim text.