Process for induction culture of cytotoxic T lymphocytes having killing activity against tumor cells
AssigneeThe Institute of Physical and Chemical Research
InventorsTadao Ohno, Shu Qin Liu, Takeshi Todoroki
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Abstract
A process for an induction culture of a cytotoxic T lymphocyte having cell killing activities against a tumor cell which comprises the step of coculturing a tumor tissue containing said tumor cell and a lymphocyte of autologous peripheral blood obtained from a subject from which the tumor tissue is derived. The process is convenient since no separation and pure culture of tumor cells is required. The resulting cytotoxic T lymphocyte cells are useful for clinical treatments of tumors, e.g. adoptive immunotherapies.
Claims17
A process for inducing a culture of cytotoxic T lymphocytes having cell killing activities against a tumor cell, comprising in vitro coculturing tumor tissue containing said tumor cell with lymphocyte of autologous peripheral blood obtained from a subject from which the tumor tissue is derived, said tumor tissue being obtained from fixed and sectioned tumor tissue.
A process for expanding a culture of cytotoxic T lymphocytes having cell killing activities against a tumor cell, comprising in vitro coculturing tumor tissue containing said tumor cell and lymphocyte of autologous peripheral blood obtained from a subject from which the tumor tissue is derived, said tumor tissue being obtained from fixed and sectioned tumor tissue separating the tumor tissue from the cytotoxic T lymphocyte, and culturing the cytotoxic T lymphocyte in the absence of said tumor cells.
A process for inducing a culture of cytotoxic T lymphocytes having cell killing activities against a tumor cell, comprising coculturing tumor tissue containing said tumor cell with a lymphocyte of autologous peripheral blood obtained from a subject from which the tumor tissue is derived, said tumor tissue being obtained from frozen and sectioned tumor tissue.
A process for expanding a culture of cytotoxic T lymphocytes having cell killing activities against a tumor cell, comprising in vitro coculturing tumor tissue containing said tumor cell and lymphocyte of autologous peripheral blood obtained from a subject from which the tumor tissue is derived, said tumor tissue being obtained from frozen and sectioned tumor tissue separating the tumor tissue from the cytotoxic T lymphocyte, and culturing the cytotoxic T lymphocyte in the absence of said tumor cells.
A process for inducing a culture of cytotoxic T lymphocytes having cell killing activities against a tumor cell, comprising coculturing tumor tissue containing said tumor cell with a lymphocyte of autologous peripheral blood obtained from a subject from which the tumor tissue is derived, said tumor tissue being obtained from fixed and minced tumor tissue.
A process for expanding a culture of cytotoxic T lymphocytes having cell killing activities against a tumor cell, comprising in vitro coculturing tumor tissue containing said tumor cell and lymphocyte of autologous peripheral blood obtained from a subject from which the tumor tissue is derived, said tumor tissue being obtained from fixed and minced tumor tissue separating the tumor tissue from the cytotoxic T lymphocyte, removing and culturing the cytotoxic T lymphocyte in the absence of said tumor cells.
Dependencies are inferred from the claim text.