Humanized green fluorescent protein genes and methods
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsSergei Zolotukhin, Nicholas Muzyczka, William W. Hauswirth
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Abstract
Disclosed are synthetic and "humanized" versions of green fluorescent protein (GFP) genes adapted for high level expression in mammalian cells, especially those of human origin. Base substitutions are made in various codons in order to change the codon usage to one more appropriate for expression in mammalian cells. Recombinant vectors carrying such humanized genes are also disclosed. In addition, various methods for using the efficient expression of humanized GFP in mammalian cells and in animals are described.
Claims81
A humanized green fluorescent protein (GFP) gene.
An expression vector comprising a humanized GFP reporter gene operatively positioned downstream from a promoter, the promoter directing expression of the humanized GFP gene in a mammalian cell.
A recombinant host cell comprising a humanized GFP gene.
A reporter gene expression kit comprising, in suitable container means, an expression vector comprising a humanized GFP gene.
Dependencies are inferred from the claim text.