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US 5,874,226 AGranted
In situ immunodetection of antigens
AssigneeH. Lee Browne
InventorsFusun N. Zeytinoglu, Franz B. Thiebaut
Life of this patent
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Abstract
An antibody targeted to an antigen is brought into contact with a body component in situ. The resulting antibody/antigen complex is labeled and may be amplified. The label is then detected either in situ or ex situ.
Claims44
A method for detecting a target antigen proximally associated with a body component comprising:
- providing a first antibody which binds to the target antigen;
- contacting a portion of the body component in situ with the first antibody;
- allowing the first antibody to bind with the antigen to form an antibody/antigen complex;
- labeling the antibody/antigen complex;
- and
- detecting the label wherein the presence of the label indicates the presence of a target antigen.
3 more elements
A method for detecting a target antigen on the skin of a patient comprising:
- providing a labeled first antibody which selectively binds to the target antigen;
- placing a retainer in juxtaposed relation to the skin to define a work space;
- contacting the skin with the first antibody within the work space;
- allowing the first antibody to bind with the antigen to form an antibody/antigen complex;
- amplifying the label;
- and
- detecting the label wherein the presence of the label indicates the presence of a target antigen.
4 more elements
An in situ immunodetection kit for detecting a target antigen proximally associated with a section of skin, the kit comprising:
- a retainer which can be affixed to the skin to define a testing area;
- a labeled antibody solution which binds to the target antigen at the testing area to form an antibody/antigen complex;
- and
- reagents for detecting the label.
1 more element
An in situ kit for detecting a disease condition of a body comprising:
- an antibody which selectively binds to a target antigen proximally associated with a component of the body;
- a retainer removably affixed to the body and defining a work space in which the antibody is applied;
- a label which is coupled to the first antibody to provide a signal;
- and
- chemical amplification of the signal.
2 more elements
Dependencies are inferred from the claim text.