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US 5,872,003 AGranted
DNA sequencing by mass spectrometry via exonuclease degradation
AssigneeSequenom, Inc.
InventorHubert Koster
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Abstract
Methods for determining the sequence of nucleic acids by cleaving the nucleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectrometry, and determining the sequence of the nucleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simulataneously determining more than one nuleic acid sequence.
Claims21
A system for exonuclease-mediated mass spectrometric sequencing comprising:
- (i) a reactor and capillary tube means for performing and containing exonuclease cleavage reactions of the target nucleic acids, the cleavage reaction forming a train of sequentially released individual nucleotides;
- (ii) mass spectrometric means for detecting the train of individual nucleotides released by the exonuclease cleavage of the target nucleic acid;
- and
- (iii) transfer means for transferring the train of individual nucleotides from the reactor means to the mass spectrometric means.
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